Bacteria morphology lab2
1 lab 2 - microbial enumeration i introduction viable counts and total counts are two basic methods to enumerate bacteria a lot of studies have been done showed the different advantage and disadvantage of them. This laboratory manual contains exercises designed for a semester- or quarter-long course in introductory microbiology, for non-biology majors as such, it assumes no previous laboratory experience in biology and no college-level coursework in biology exercises are designed to teach critical laboratory skills through hands-on experience, including compound microscopy. Bacterial colony morphology bacteria grow on solid media as colonies a colony is defined as a visible mass of microorganisms all originating from a single mother cell, therefore a colony constitutes a clone of bacteria all genetically alike in the identification of bacteria and fungi much.
About the bacteria other than morphology and arrangement the gram stain is a differential stain use the focusing procedure in lab #2 to view the smear using the 100x (oil immersion lens) focus line e coli staining and how it differentiates bacteria on the basis of their cell wall structure should also be included. An investigation of bacterial internal and external structures from dr klein. The bacteria is negatively charged so a basic dye will attract better to it why is the negative staining procedure better than the simple stain when observing morphologies the negative stain does not stain the bacteria, but only the surrounding area. Lab 2: bacterial cell morphology by mariam kaleem on prezi prezicom gram-positive bacteria have a thick mesh-like cell wall made of peptidoglycan (50–90% of cell envelope), and as a result are stained purple by crystal violet, whereas gram-negative bacteria have a thinner layer (10% of cell envelope), so do not retain the purple stain and. Appendix a – example answers to study questions safety and introduction example answers to study questions bacterial cell morphology study questions 1 what are the most common shapes of bacterial cells aseptic technique is used to prevent contamination of the bacterial cultures and media used in lab 2 the incinerator is used to.
Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives the procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction. Start studying bacte lab 1- bacterial morphology and types learn vocabulary, terms, and more with flashcards, games, and other study tools morphology of bacteria that requires a special stain or microscope to identify bacillus bacte lab 2- structure and composition of microorganisms. Concept map for lab 2: terminology colony morphology and pigmentation a colony is a visible mass of microorganisms growing on an agar surface and usually originating from a single organism or arrangement of organisms. Lab report of microbiology 3 firstly introduced by cristian gram in 1883this method is used to distinguish between gram positive and gram-negative bacteria which have consistent differences in their cell walls gram-positive bacteria stain blue-purple and gram-negative bacteria stain pink- red there are several objective why we should do. The morphology of the gram positive bacteria was telling because c xerosis was the only bacteria with diphlococcus morphology the gram negative bacteria appeared to be bacillus the purpose of the tsa aerobic and anaerobic plates was to determine whether or not the bacteria grew in these conditions.
Bacteria morphology lab2
- Bacteria exist in a variety of shapes and sizes for example, individual escherichia coli cells are rod-shaped with an average length of 2 μm and width of 05 μm while streptococcus cells are spherical with an average diameter of 1 μm some bacteria (such as e coli) exist as single cells while others form distinct patterns of association.
- 12 don’t leave media open to the air – bacteria and fungi in the air can contaminate the media 13 don’t over-inoculate it doesn’t take much inoculum to start a culture simply touch the loop or needle to the bacterial growth and obtain a small amount on the loop don’t.
Start studying bacterial morphology and staining techniques lab, microbiology learn vocabulary, terms, and more with flashcards, games, and other study tools. Essentials of laboratory microbiology author(s): lisa shimeld edition: 2 bacterial morphology ii aseptic techniques and the isolation of bacteria lab 1: aseptic methods identification of unknown bacteria lab 47: 1st unknown lab 48: 2nd unknown. Viewing prepared slides of bacterial morphology disinfecting the workspace, using live cultures and cells such as a cheek smear, teeth plaque and yeast were examined via wet mount staining was done to all of these cultures with a gram stain and as well as a congo red stain.